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Abstract Human mesenchymal stem cells (hMSCs) have gained traction in transplantation therapy due to their immunomodulatory, paracrine, immune‐evasive, and multipotent differentiation potential. The inherent heterogeneity of hMSCs poses a challenge for therapeutic treatments and necessitates the identification of robust biomarkers to ensure reproducibility in both in vivo and in vitro experiments. In this study, we utilized dielectrophoresis (DEP), a label‐free electrokinetic phenomenon, to investigate the heterogeneity of hMSCs derived from bone marrow (BM) and adipose tissue (AD). The electrical properties of BM‐hMSCs were compared to homogeneous mouse fibroblasts (NIH‐3T3), human fibroblasts (WS1), and human embryonic kidney cells (HEK‐293). The DEP profile of BM‐hMSCs differed most from HEK‐293 cells. We compared the DEP profiles of BM‐hMSCs and AD‐hMSCs and found that they have similar membrane capacitances, differing cytoplasm conductivity, and transient slopes. Inducing both populations to differentiate into adipocyte and osteoblast cells revealed that they behave differently in response to differentiation‐inducing cytokines. Histology and reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) analyses of the differentiation‐related genes revealed differences in heterogeneity between BM‐hMSCs and AD‐hMSCs. The differentiation profiles correlate well with the DEP profiles developed and indicate differences in the heterogeneity of BM‐hMSCs and AD‐hMSCs. Our results demonstrate that using DEP, membrane capacitance, cytoplasm conductivity, and transient slope can uniquely characterize the inherent heterogeneity of hMSCs to guide robust and reproducible stem cell transplantation therapies. 

Abstract Infrared (IR) luminosity of lightning channel in the 3–5 μm range usually persisted throughout the entire interstroke interval, which is in contrast to the simultaneously recorded visible (0.4–0.8 μm) luminosity that always decayed to an undetectable level prior to a subsequent return stroke pulse. A longer visible luminosity period at the end of flash tended to be associated with a longer IR afterglow period following the decay of visible luminosity (and by inference current) to an undetectable level. At the end of flash, the IR luminosity persisted up to about 1 s, and the median IR afterglow duration was a factor of 10 longer than the median visible luminosity duration. The IR luminosity often exhibited a hump when the visible luminosity was monotonically decaying or undetectable, with the corresponding channel temperature being likely around 3400 K. 

Abstract Our basic knowledge of downward positive lightning leaders is incomplete due to their rarity and limited ability of VHF mapping systems to image positive streamers. Here, using high‐speed optical records and wideband electric field and magnetic field derivative signatures, we examine in detail the development of a descending positive leader, which extended intermittently via alternating branching at altitudes of 4.2 to 1.9 km and involved luminosity transients separated by millisecond‐scale quiet intervals. We show that the transients (a) are mostly initiated in previously created but already decayed branches, at a distance of the order of 100 m above the branch lower extremity, (b) extend bidirectionally with negative charge moving up, (c) establish a temporary (1 ms or so) steady‐current connection to the negative part of the overall bidirectional leader tree, and (d) exhibit brightening accompanied by new breakdowns at the positive leader end. One of the transients unexpectedly resulted in a negative cloud‐to‐ground discharge. Both positive and negative ends of the transients extended at speeds of 10⁶–10⁷ m/s, while the overall positive leader extension speed was as low as 10³–10⁴ m/s. Wideband electric field signatures of the transients were similar to K‐changes, with their millisecond‐ and microsecond‐scale features being associated with the steady current and new breakdowns, respectively. For transients with both ends visible in our optical records, charge transfers and average currents were estimated to be typically a few hundreds of millicoulombs and some hundreds of amperes, respectively. 

Oxygen isotopes (δ18O) are the most commonly utilized speleothem proxy and have provided many foundational records of paleoclimate. Thus, understanding processes affecting speleothem δ18O is crucial. Yet, prior calcite precipitation (PCP), a process driven by local hydrology, is a widely ignored control of speleothem δ18O. Here we investigate the effects of PCP on a stalagmite δ18O record from central Vietnam, spanning 45 – 4 ka. We employ a geochemical model that utilizes speleothem Mg/Ca and cave monitoring data to correct the δ18O record for PCP effects. The resulting record exhibits improved agreement with regional speleothem δ18O records and climate model simulations, suggesting that the corrected record more accurately reflects precipitation δ18O (δ18Op). Without considering PCP, our interpretations of the δ18O record would have been misleading. To avoid misinterpretations of speleothem δ18O, our results emphasize the necessity of considering PCP as a significant driver of speleothem δ18O. 

Abstract This review covers selected results of recent observations of lightning discharges performed across the entire electromagnetic spectrum (radiofrequency, optical, and energetic radiation) at the Lightning Observatory in Gainesville, Florida. The most important results include (a) characterization of the preliminary-breakdown, stepped-leader, and return-stroke processes in high-intensity (⩾50 kA) negative lightning discharges, (b) the first high-speed video images of bidirectional leader that made contact with the ground and produced a return stroke, (c) discovery of negative stepped leader branches colliding with the lateral surface of neighboring branches of the same leader, (d) new data on the occurrence context and properties of compact intracloud discharges, and (e) observation of a terrestrial gamma-ray flash that occurred during a bipolar cloud-to-ground lightning discharge. The results serve to improve our understanding of the physics of lightning with important implications for lightning modeling, lightning protection, and high-energy atmospheric physics studies. 

Most paleoclimate studies of Mainland Southeast Asia hydroclimate focus on the summer monsoon, with few studies investigating rainfall in other seasons. Here we present a multiproxy stalagmite record (45,000 – 4,000 years) from central Vietnam, a region that receives most of its annual rainfall in autumn (September-November). We find evidence of a prolonged dry period spanning the last glacial maximum that is punctuated by an abrupt shift to wetter conditions during the deglaciation at ~14ka. Paired with climate model simulations, we show that sea level change drives autumn monsoon rainfall variability on glacial-orbital timescales. Consistent with the dry signal in the stalagmite record, climate model simulations reveal that lower glacial sea level exposes land in the Gulf of Tonkin and along the South China Shelf, reducing convection and moisture delivery to central Vietnam. When sea level rises and these landmasses flood at ~14ka, moisture delivery to central Vietnam increases causing an abrupt shift from dry to wet conditions. On millennial timescales, we find signatures of well-known Heinrich Stadials (dry conditions) and Dansgaard-Oeschger Events (wet conditions). Model simulations show that during the dry Heinrich Stadials, changes in sea surface temperature related to meltwater forcing cause the formation of an anomalous anticyclone in the Western Pacific, which advects dry air across central Vietnam decreasing autumn rainfall. Notably, sea level modulates the magnitude of millennial-scale dry and wet phases by muting dry events and enhancing wet events during periods of low sea level, highlighting the importance of this mechanism to autumn monsoon variability.